Comparison of the activity and pluripotency maintaining potential of human leukemia inhibitory factor (LIF) produced in E.coli and CHO cells
نویسندگان
چکیده
Introduction Leukemia inhibitory factor (LIF) is a polyfunctional cytokine with numerous regulatory effects in vivo and in vitro. In murine stem cell cultures it is the essential media supplement for the maintenance of pluripotency of embryonic and induced pluripotent stem cells. To explore if the glycosylation and/or other post-translational modifications are affecting this activity, we produced and isolated LIF from either eucaryotic cells (Chinese hamster ovary (CHO) cells) or procaryotes (E. coli) and compared their biological activities in this study.
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